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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: A robust cell culture system for large scale feeder cell-free expansion of human breast epithelial progenitors

Fig. 4

EpiProPlus medium maintains breast epithelial progenitor cell characteristics in cultures. a Average number of days between passages of luminal progenitors (CD49lowEpCAMbright) grown in EpiProPlus medium. b Average number of days between passages of bipotent (CD49lowEpCAMbright) progenitor grown in EpiProPlus medium. c Representative photomicrographs and quantification of ERα+ cells in expanded luminal progenitor before ex vivo culturing (passage 0) and after five passages (DAPI nuclear staining in blue, ER staining in red). d Representative photomicrographs of colonies from ex vivo expanded luminal and bipotent progenitors immunofluoroscently stained with CK14 and CK8/18 antibodies. Epithelial to mesenchymal transition (EMT) and epithelial specific gene expression in expanded luminal (e) and bipotent (f) progenitors over subsequent passages. All results are presented as mean ± SEM from three biological replicates. Bars represent 200 μm (c), or crystal violet pictures 1000 μm and immunofluorescent pictures 400 μm (d). Cadh cadherin, CK cytokeratin, ERα estrogen receptor alpha, P passage, Vim vimentin. *p < 0.05, **p < 0.005, ***p < 0.0001

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