Fig. 3

Molecular phenotype characterization of LNCs. a P0 limbal niche cells (LNCs) were double-stained for Vim (red fluorescence) and CK12 (green fluorescence), ΔNp63α (green fluorescence) or Pax6 (green fluorescence). b P3 LNCs grown in MESCM (ME-LNCs) and LNCs grown in DMEM/F12 + 10% FBS (DF-LNCs) were double-stained for Vim (red fluorescence) and CK12 (green fluorescence), ΔNp63α (green fluorescence) or Pax6 (green fluorescence). Furthermore, they were single-stained for N-cadherin, Oct4, or Sox2 (scale bar = 100 μm). c Relative mRNA levels of Oct4 and Sox2 expressed in P3 DF-LNCs and ME-LNCs. d Western blot analysis of Oct4 and Sox2 expression in P3 DF-LNCs and ME-LNCs; GAPDH was used as a loading control. **p < 0.01