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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Rat limbal niche cells can induce transdifferentiation of oral mucosal epithelial cells into corneal epithelial-like cells in vitro

Fig. 4

Three-dimensional coculturing of oral mucosal epithelial cells (OMECs) and limbal niche cells (LNCs) under different conditions. a Morphologic characterization of OMECs and LNCs cocultured on 3D Matrigel. b The relative mRNA levels of CK12 and Pax6 in cocultured OMECs and LNCs. c The immunofluorescence of suspended OMECs and LNCs cocultured on 3D Matrigel. Cells were single-stained for CK12 or Pax6 (red fluorescence), and nuclei were stained by DAPI. Scale bar = 100 μm. d The positive rate of CK12 and Pax6 expression in cocultured OMECs and LNCs. **p < 0.01, *p < 0.05. DF-ME LNCs grown in DMEM/F12 (DF-LNCs) 3D cocultured in MESCM, DF-SH DF-LNCs 3D cocultured in SHEM, ME-ME LNCs grown in MESCM (ME-LNCs) 3D cocultured in MESCM, ME-SH ME-LNCs 3D cocultured in SHEM

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