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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Rat limbal niche cells can induce transdifferentiation of oral mucosal epithelial cells into corneal epithelial-like cells in vitro

Fig. 5

Transwell coculturing of oral mucosal epithelial cells (OMECs) and limbal niche cells (LNCs) in supplemented hormonal epithelial medium (SHEM). a Morphologic characterization of OMECs and LNCs cocultured in Transwell. Scale bar = 100 μm. b The relative mRNA expression of CK12 and Pax6 in cocultured OMECs and LNCs. c The relative protein levels of cocultured OMECs and LNCs. d Western blot analysis of CK12 and Pax6 expression in cocultured OMECs and LNCs; β-actin was used as an internal control. e Cells were single-stained for CK3, CK12, Pax6, and ΔNp63α, and nuclei were stained by DAPI. Scale bar = 50 μm. **p < 0.01, *p < 0.05. DF Cocultured OMECs supported by DF-LNCs in Transwell, LEPC Limbal epithelial progenitor cell, ME Cocultured OMECs supported by ME-LNCs in Transwell

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