Fig. 2From: Organ culture of seminiferous tubules using a modified soft agar culture systemImmunofluorescence staining of colonies following 40 days of culture. Immunocytochemistry of colonies was performed by specific antibodies for different markers of germ cell development by immunofluorescence, including INTEGRIN α6, PLZF, ACROSIN (ACR), SCP3, and VIMENTIN. Scale bars =150 μm. PI propidium iodideBack to article page