Fig. 3From: Organ culture of seminiferous tubules using a modified soft agar culture systemImmunofluorescence staining of frozen longitudinal sections of seminiferous tubules following 40 days of culture. Immunohistochemistry staining of frozen STs sections was performed by specific antibodies for different markers of germ cell development by immunofluorescence, including INTEGRIN α6, PLZF, ACROSIN (ACR), SCP3, and VIMENTIN. Scale bars =150 μm. PI propidium iodideBack to article page