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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Gene expression profiling of chondrogenic differentiation by dexamethasone-conjugated polyethyleneimine with SOX trio genes in stem cells

Fig. 2

Evaluation of cytotoxicity, uptake efficiency of nanoparticles (NPs), and expression levels of delivered genes. a, b Assessment of the cytotoxicity of trio-coated polyethyleneimine (PEI) and differentiation-inducing nanoparticles (DI-NPs) by CCK8 and Live/Dead assays in hMSCs transfected with nothing (panel a), trio-coated PEI (panel b), and DI-NPs (panel c). c, d Assessment of the cellular uptake of trio-coated PEI (panel a) and DI-NPs (panel b) into hMSCs over time by FACS and fluorescence microscopy. e RT-PCR and Western blot analysis of SOX5, SOX6, and SOX9 in hMSCs transfected with nothing (column a), trio-coated PEI (column b), and DI-NPs (column c). f Confocal microscopy analysis of SOX5, SOX6, and SOX9 in hMSCs transfected with PEI and DI-NPs complexed with SOX5 (panels a and e), SOX6 (panels b and f), SOX9 (panels c and g), and merged images (panels d and h). GFP green fluorescent protein, YFP yellow fluorescent protein

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Stem Cell Research & Therapy

ISSN: 1757-6512