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Scheme 1 | Stem Cell Research & Therapy

Scheme 1

From: Gene expression profiling of chondrogenic differentiation by dexamethasone-conjugated polyethyleneimine with SOX trio genes in stem cells

Scheme 1

Schematic diagram of the preparation of DI-NPs to induce chondrogenesis of hMSCs. A Dexamethasone (DEX)/tetramethylrhodamine (TRITC)-conjugated branched polyethyleneimine (bPEI) (DEX/PEI) is made by the serial conjugation of bPEI and DEX. The DEX/PEI is complexed with SOX trio genes (SOX5, SOX6, and SOX9), called differentiation inducing nanoparticles (DI-NPs). B Transfection of DI-NPs induces chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The DI-NPs enter hMSCs via endocytosis. The plasmid DNAs (pDNAs) are released from DI-NPs into the cytosol and the expression levels of specific genes, related to chondrogenesis, are changed (up and down); finally, chondrogenic differentiation of hMSCs is induced

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Stem Cell Research & Therapy

ISSN: 1757-6512