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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Exosomes derived from miR-92a-3p-overexpressing human mesenchymal stem cells enhance chondrogenesis and suppress cartilage degradation via targeting WNT5A

Fig. 1

Characterization of human mesenchymal stem cells (MSCs) and exosomes. a MSCs exhibited a representative spindle-like morphology. b MSCs exhibited multidifferentiation capacity for osteogenesis, adipogenesis, and chondrogenesis. c Flow cytometric analysis of mesenchymal positive markers, such as CD73, CD90, and CD105, and negative markers, such as CD11b, CD19, CD34, CD45, and HLA-DR. Blue histograms represent the isotype controls, and the red solid peak represents the marker indicated. This experiment was repeated three times independently. d-e Particle size distribution of exosomes was measured by Nanosight. f Morphology of exosomes observed by transmission electron microscopy (TEM). g Exosome surface markers (CD9, CD63, CD81, and HSP70) measured using western blotting. This experiment was repeated three times independently, and representative results are shown

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Stem Cell Research & Therapy

ISSN: 1757-6512