Fig. 3

a Expression of miR-92a-3p and WNT5A mRNA in MSC -chondro-differentiated-Exos. MSCs were induced to undergo chondrogenesis with TGF-β3 for 3, 7, 14, 21, and 28 days as indicated (solid lines). Gene expression levels of miR-92a-3p (A) and WNT5A (B) in chondro-differentiated MSC-Exos and WNT5A (C) in chondro-differentiated MSCs were determined by qRT-PCR. MSCs cultured without TGF-β3 at corresponding time points served as negative controls (broken lines). Quantitative data are presented as means ± standard deviations of three independent experiments. U6 and GAPDH were used as endogenous controls. Data are presented as means ± standard deviations of six samples. *P < 0.05, **P < 0.01, ***P < 0.001. b MSC-miR-92a-3p-Exos regulate cartilage-specific gene expression during chondrogenesis. MSCs were treated with TGF-β3 to induce chondrogenesis and treated with MSC-miR-92a-3p-Exos or MSC-anti-miR-92a-3p-Exos. Expression levels of miR-92a-3p (A, I) were determined by qRT-PCR. Expression levels of aggrecan (B, J, Q), COL2A1 (C, K, Q), SOX9 (D, L, Q), COL10A1 (E, M), RUNX2 (F, N, Q), MMP13 (G, O, Q), and WNT5A (H, P, Q) were determined by both qRT-PCR and western blotting. U6 and GAPDH were used as endogenous controls. Data represented as means ± standard deviations of six samples. **P < 0.01, ***P < 0.001