Fig. 4

Expression of miR-92a-3p and WNT5A in normal and OA cartilage. Relative miR-92a-3p and WNT5A (a, b) mRNA levels in normal and OA cartilage-secreted exosomes and (c) WNT5A mRNA levels in normal and OA cartilage tissues were determined by qRT-PCR. U6 and GAPDH were used as endogenous controls. Each dot represents a value from a single experiment involving a single donor. The bar shows the mean and 95% confidence intervals of the values from six different donors per group. **P < 0.01, ***P < 0.001. WNT5A protein levels in normal cartilage and OA cartilage-secreted exosomes or tissues were determined by western blotting and immunohistochemistry using anti-WNT5A monoclonal antibody (d, e). GAPDH was used as an endogenous control. Data shown are representative of results of six normal and OA cartilage samples. Scale bar 50 μm