Fig. 6

PHCs were transfected with siWNT5A. a The gene expression levels of aggrecan, COL2A1, COMP, COL9A1, SOX9, RUNX2, COL10A1, MMP13, and WNT5A were determined by qRT-PCR. Quantitative data are presented as means ± standard deviations of three independent experiments. GAPDH was used as an internal control. *P < 0.05, **P < 0.01, ***P < 0.001. b Expression of WNT5A, RUNX2, MMP13, SOX9, COL2A1, and aggrecan as visualized by western blot. GAPDH was used as an internal control. c Alignment of miR-92a-3p and the 3′-UTR of WNT5A, a potential miR-92a-3p target. d, e A luciferase reporter carrying the 3’-UTR of wild-type (Luc-WNT5A-UTR) or mutant (Luc-WNT5A-UTR-mut) WNT5A was introduced into 293 T cells along with negative miR-control (NC) or miR-92a-3p. Cells were harvested 48 h later for luciferase assays. Quantitative data are presented as means ± standard deviations of three independent experiments. ***P < 0.001