Fig. 7

MSC-miR-92a-3p-Exos inhibits cartilage degradation. a. Sections of femoral condyle (n = 10 per group) were stained using Safranin-O and Fast Green and were stained the miR-92a-3p probe for analysis of miR-92a-3p expression. Femoral condyle sections (n = 10 per group) stained with col2a1, aggrecan, wnt5a, or MMP13 as primary antibodies (scale bar: 50 μm). b. Gene expression levels of miR-92a-3p (A), WNT5A (B), MMP13 (C), COL2A1 (D), and aggrecan (E) were estimated by qRT-PCR. Quantitative data are presented as means ± standard deviations of three independent experiments. GAPDH was used as an internal control. *P < 0.05, **P < 0.01, ***P < 0.001. (F) Protein expression levels of aggrecan, col2a1, MMP13, and Wnt5a were visualized by western blotting. GAPDH was used as an endogenous control