Fig. 4

In vitro effect of TSG-6 on caerulein- and LPS-stimulated primary pancreatic acinar cells (PACs). a TSG-6 mRNA expression levels in hAT-MSCs transfected with TSG-6 siRNA (TSG-6 siRNA MSCs), hAT-MSCs transfected with control siRNA (scr siRNA MSCs), and naive hAT-MSCs (MSCs). b, c Caerulein- and LPS-stimulated primary PACs were indirectly co-cultured with each of the three hAT-MSC types for 12 h. b Grp78, CHOP, and caspase-12 mRNA expression levels in primary PACs. c Grp78, CHOP, and caspase-12 protein expression levels. d The expression levels of NF-κB p65 activity were analysed by western blotting. e TUNEL staining in PACs in each group observed at ×200 magnification. TUNEL staining revealed the number of cells undergoing apoptosis or pyroptosis. Results are presented as the mean ± SD obtained from three independent experiments ^*P < 0.05, ^**P < 0.01. CHOP CCAAT-enhancer-binding protein homologous protein, Grp7 78-kDa glucose-regulated protein, MSC mesenchymal stem cells, PBS phosphate-buffered saline, SAP severe acute pancreatitis, scr scrambled, TSG-6 tumour necrosis factor-α-induced gene/protein 6