Fig. 6

Beneficial effects of hAT-MSCs depend on TSG-6 secretion due to ER stress and NF-κB activity. a, b The mRNA and protein expression levels of Grp78, CHOP, and caspase-12 were analysed by qRT-PCR and western blotting, respectively. The band intensity on films is presented as the relative ratio of Grp78, CHOP, and caspase-12 to the band intensity of β-actin. c The expression levels of TNF-α and activity of p65 were analysed by western blotting. The band intensity on films is presented as the relative ratio of the level of TNF-α to that of β-actin; for NF-κB signalling, p65 activity was presented in the form of the ratio of phosphorylated p65 to p65. Results are presented as the mean ± SD obtained from three independent experiments ^*P < 0.05, ^**P < 0.01. CHOP CCAAT-enhancer-binding protein homologous protein, Grp7 78-kDa glucose-regulated protein, MSC mesenchymal stem cells, PBS phosphate-buffered saline, SAP severe acute pancreatitis, scr scrambled, TSG-6 tumour necrosis factor-α-induced gene/protein 6