Fig. 3

Differential reduction in reactive oxygen species (ROS) levels by tissue-specific mesenchymal stem cells (MSCs). (a) Representative flow cytometry data depicting mean fluorescence intensity (MFI) of ROS values indicated in arbitrary units in MitoSOX red–stained untreated and antimycin-treated cardiomyocytes, co-cultured with tissue-specific MSCs (left panel). (b) Bar graphs depicting reduction in ROS for bone marrow (BM)-MSCs, adipose (AD)-MSCs, dental pulp (DP)-MSCs, and Wharton’s jelly (WJ)-MSCs when co-cultured with stressed cardiomyocytes (right panel) determined by flow cytometry. Data are expressed as mean ± standard error of the mean (SEM) (n = 4), *P <0.05, **P <0.01, ***P <0.001 by one-way analysis of variance (ANOVA) followed by post hoc Tukey analysis. (c) Correlation between reduction in mitochondrial ROS (mtROS) reduction and mitochondrial transfer percentages (percentage double-positive cells) in cardiomyocyte co-cultures determined by Pearson’s correlation (P <0.05). Abbreviation: ns non-significant