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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stromal cells from myelodysplastic and acute myeloid leukemia patients display in vitro reduced proliferative potential and similar capacity to support leukemia cell survival

Fig. 2

MDS-MSCs and AML-MSCs show typical MSC biological properties. a Flow cytometry analysis of HD-MSC (N = 9), MDS-MSC (N = 3), and AML-MSC (N = 4) immunophenotype. Histograms represent percentage of cells positive for CD90, CD45, CD34, CD13, CD105, CD19, CD29, CD44, CD73, CD14, and HLA-DR. All differences not significant. b Representative microphotographs of Alizarin red (upper row) and Oil Red O (lower row) staining of HD-MSCs, MDS-MSCs, and AML-MSCs cultured for 3 weeks in osteogenic and adipogenic conditions, respectively. Magnification 10×; scale bar, 100 μm. c qRT-PCR analysis of Runx2 (Runx2/GAPDH relative levels) and PPARγ (PPARγ/GAPDH relative levels) in undifferentiated (black histograms) and differentiated (crossed histograms) cells following 3 weeks of culture. Expression levels of differentiation specific genes in undifferentiated cells taken as 1 (mean ± SEM of at least four independent experiments). *P < 0.05; **P < 0.01; ***P < 0.001. HD-MSC mesenchymal stromal cell from healthy donor, MDS-MSC mesenchymal stromal cell from myelodysplastic syndrome patient, AML-MSC mesenchymal stromal cell from acute myeloid leukemia patient, PPARγ peroxisome proliferator activated receptor gamma, RUNX2 Runt-related transcription factor 2, GAPDH glyceraldehyde 3-phosphate dehydrogenase

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