Fig. 2
Therapeutic efficacy of FE on limb salvage and blood perfusion in nude mice after critical limb ischemia. a Flowchart for treatment of hindlimb ischemia and evaluation methods. b Representative images of gross observation at 28 days post operation. c Scores of tissue damage in FEHigh group were significantly decreased at day 28. d Ratio of limb outcomes demonstrates improved limb salvage in FEHigh group. e Laser Doppler perfusion imaging (LDPI) showed dynamic changes in blood perfusion in limb ischemia of each group at days 0, 7, 14, 21, and 28 post operation. f Quantitative analysis of blood flow measured by LDPI. Ratio of LDPI in ischemic hindlimb to contralateral hindlimb calculated over observation period. Data represent mean ± SD (n = 10 per group). *p < 0.05. FE fat extract, PBS phosphate-buffered saline