Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Fat extract promotes angiogenesis in a murine model of limb ischemia: a novel cell-free therapeutic strategy

Fig. 3

Histological evaluation of necrosis and angiogenesis in ischemic hindlimb. a Hematoxylin–eosin (H&E) staining of gastrocnemius (GC) muscle of calf. Arrows indicate necrotic myofibers; arrowheads indicate regenerating myofibers characterized by central nuclei; asterisks indicate healthy myofibers with peripheral nuclei. Although H&E staining showed muscle degeneration and necrosis in all ischemic limbs, a higher degree of muscle degeneration and necrosis was observed in PBS-treated group. b Quantitative analysis of percentage area of necrotic tissue. Significantly fewer percentage areas of necrotic tissues measured in FEHigh-treated group. c Immunostaining of CD31+ capillaries in ischemic GC muscles. Arrows indicate blood vessels. FE-treated group showed more CD31+ blood vessels. d Quantitative analysis of capillary density. Significantly higher blood vessel density was measured in FEHigh-treated group. e Immunostaining of CD68 in ischemic GC muscles. Arrows indicate CD68+ inflammatory cells. f Quantification of CD68+ cell density. FEHigh-treated group showed significantly lower CD68+ inflammatory cell infiltration. *p < 0.05. FE fat extract, PBS phosphate-buffered saline

Back to article page