Fig. 1
From: Tcf7l1 directly regulates cardiomyocyte differentiation in embryonic stem cells
Genetic ablation of Tcf7l1 leads to delayed and partially blocked mesendoderm formation. a Western blot confirmation of Tcf7l1 absence in Tcf7l1−/− ESCs. b Downregulation of Oct4 and Sox2 during differentiation is impaired in Tcf7l1−/− ESCs. c Expression of mesendoderm genes, T, Eomes, Sox17, and Mesp1, is delayed and partially reduced in Tcf7l1−/− ESCs. Gene expression assayed by real-time RT-PCR. N ≥ 3; *p < 0.05 versus control cells