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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: The manner of decay of genetically defective EYS gene transcripts in photoreceptor-directed fibroblasts derived from retinitis pigmentosa patients depends on the type of mutation

Fig. 4

Detection of expression and identification of sequences of EYS gene transcripts corresponding to exon 6–11 that includes c.1211dupA in photoreceptor-directed fibroblasts from EYS-RP patients. a RT-PCR analysis of expression of the EYS gene using primer pairs corresponding to exon 6–11. Expression levels of EYS gene were clearly up-regulated by CRX, RAX, NeuroD and OTX2 transduction in Pt#3 and N#2 10 days after transduction. Y79 was used as a positive control. b Electropherogram corresponding to c.1211dupA by sequencing of RT-PCR products and the genome. Sequencing analyses revealed that these RT-PCR products corresponding to exon 6–11 consisted of normal sequences with an extremely low level of defected sequences

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Stem Cell Research & Therapy

ISSN: 1757-6512