Table 1 Primer sequences for qRT-PCR and annealing temperatures used (°C)
Factor | Forward Primer (5′ → 3′) | Reverse Primer (5′ → 3′) | °C |
|---|---|---|---|
Gap43 | GTCCACTTTCCTCTCTATTTC | TGTTCATTCCATCACATTGA | 55.0 |
Tau | GGCTACACACCAACCTTTG | CCACCTCCTCCTCACTTC | 57.2 |
Rac1 | TCATCAGTTACACGACCAATG | ACGCAGTCTGTCATAATCTTC | 56.8 |
RhoA | CACACAAGGCGGGAGTTAG | CGTCTTTGGTCTTTGCTGAAC | 60.5 |
β-actin | ACTATC GGCAATGAGCGGTTC | AGAGCCACCAATCCACACAGA | 65.0 |