Fig. 7

Effects of transplantation of N-ADSCs on blood flow and histological staining. a Representative color laser Doppler images of the superficial blood flow in the denervated hindlimbs were acquired on days 0, 7, and 28 post-transplantation. b The blood perfusion index was significantly higher in the N-ADSCs group as compared to that in the ADSCs and PBS control groups over 4 weeks. c Representative fluorescent staining for CD31 (red), ADSCs (green), and nuclei with DAPI (blue) in the PBS, ADSC, and N-ADSC groups. d Representative immunohistochemical staining for CD31 in the PBS, ADSC, and N-ADSCs groups. e Statistical analysis indicated that the survived ADSCs were significantly higher in N-ADSCs than in ADSCs (P < 0.05). f Quantitative analysis revealed that microvessel densities were significantly higher in the N-ADSCs than in the ADSCs and PBS control groups (P < 0.01). Scale bar = 100 μm; *P < 0.05, **P < 0.01. ADSC, adipose-derived stem cell; PBS, phosphate-buffered solution; N-ADSC, Netrin-1-transfected ADSC