Fig. 9

Signaling pathway of Netrin-1 on ADSCs in vitro and in vivo. a Western blotting of P-AKT, AKT, P-PI3K, PI3K, P-P38, P38, P-eNOS, eNOS, P-NF-κB, NF-κB, JNK, and ERK1/2 in vitro. b Statistical analysis demonstrated that the phosphorylation of the signaling pathway of PI3K/AKT/eNOS/P-38/NF-κB was highly upregulated in the N-ADSCs group as compared to the ADSCs group in vitro, but not JNK or ERK1/2. c Western blotting of P-AKT, AKT, P-PI3K, PI3K, P-P38, P38, P-eNOS, eNOS, P-NF-κB, NF-κB, JNK, and ERK1/2 in vivo. d Statistical analysis demonstrated that the phosphorylation of the signaling pathway of PI3K/AKT/eNOS/P-38/NF-κB was highly upregulated in the N-ADSCs group as compared to the ADSCs group in vivo, but not JNK or ERK1/2; *P < 0.05. ADSC, adipose-derived stem cell; N-ADSC netrin-1-transfected ADSC