Fig. 6

The migration of HUVEC after removing the effects of DBMSCs and H₂O₂. HUVEC were initially cultured with DBMSC with 100 μM H₂O₂ for 48 h and then used in a migration assay using the xCELLigence real-time cell analyzer. After 24 h, the migration of HUVEC pretreated with H₂O₂ alone (HUVEC + H₂O₂) or with H₂O₂ and CMDBMSC (CMDBMSC + H₂O₂) significantly reduced as compared to untreated HUVEC while the migration of HUVEC pretreated with H₂O₂ and with SFDBMSC (SFDBMSC + H₂O₂) or H₂O₂ and ICDBMSC (ICDBMSC + H₂O₂) increased, but not significantly (P > 0.05). After 24 h, the migration of HUVEC pretreated with H₂O₂ and with SFDBMSC (SFDBMSC + H₂O₂) or with ICDBMSC (ICDBMSC + H₂O₂) significantly increased as compared to HUVEC pretreated with H₂O₂ (HUVEC + H₂O₂). After 24 h and as compared to untreated HUVEC, the migration of HUVEC treated with CMDBMSC or SFDBMSC did not significantly change, P > 0.05. In contrast, the migration of HUVEC treated with ICDBMSC significantly increased as compared to untreated HUVEC after 24 h. After 24 h and as compared to HUVEC treated with CMDBMSC, SFDBMSC, or ICDBMSC, the migration of HUVEC treated with H₂O₂ in the presence of CMDBMSC or SFDBMSC or ICDBMSC did not significantly change, P > 0.05. Each experiment was performed in triplicate and repeated with five independent HUVEC (passages 3–5) and DBMSC (passage 3) preparations. *P < 0.05. Bars represent standard errors