Fig. 9
The flow cytometric analysis of monocytes (THP-1) expression of ICAM-1, VCAM-1, and CD44. THP-1 were initially cultured with 100 μM H2O2 (TTHP-1) for 24 h and then cultured with DBMSCs at 5:1 THP-1:DBMSC ratio (TTHP-1/DBMSC). The flow cytometry was then evaluated after 24 h culture. The expression of ICAM-1 by TTHP-1 and by TTHP-1/DBMSC significantly increased as compared with H2O2-untreated THP-1 (a). As compared to TTHP-1, the expression of ICAM-1 by TTHP-1/DBMSC significantly increased (a). As compared with untreated THP-1, TTHP-1 expression of VCAM-1 was not significantly changed (P > 0.05) while significantly increased by TTHP-1/DBMSC (b). As compared with TTHP-1, TTHP-1/ DBMSC expression of VCAM-1 significantly increased (b). TTHP-1 and TTHP-1/DBMSC expression of CD44 significantly increased as compared to untreated THP-1 while TTHP-1/DBMSC expression of CD44 was not significantly changed as compared with TTHP-1 (c). Each experiment was performed in triplicate and repeated for five times with five independent preparations of DBMSCs and HUVEC. *P < 0.05. Bars represent standard errors