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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats

Fig. 1

miR-9 modified BMSCs (pri-miR-9-BMSCs) alleviate SAP. a BMSCs infected by pri-miR-9 and empty virus expressing GFP. b The recombinant pri-miR-9-1-PCDH plasmid was identified by dual-enzyme digestion. c pri-miR-9-1 sequence was amplified from pri-miR-9-1 plasmid using special primers. d, e The expression of mature miR-9 in pri-miR-9-BMSCs was higher than in empty virus BMSCs (EV-BMSCs) by gPCR and qRT-PCR. Data are shown as the mean ± SD for at least 3 separate experiments. ###p < 0.001, compared with EV-BMSCs by paired t test, gPCR, General PCR, qRT-PCR, quantitative real-time PCR. f, g, h, i pri-miR-9-BMSCs significantly reduced pancreatic edema, infiltration, hemorrhage and necrosis, the release of serum amylase, lipase and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and increased anti-inflammatory cytokines (IL-4, IL-10, and TGF-β) compared with SAP, SAP+PBS, BMSCs, EV-BMSCs and TuD-BMSCs groups. Data are shown as the mean ± SD for at least three separate experiments. %p < 0.05,%%p < 0.01, %%%p < 0.001, compared with NC, **p < 0.01 and ***p < 0.001, compared with NC, @@p < 0.01 and @@@p < 0.001, compared with SAP, &&p < 0.01 and &&&p < 0.001, compared with PBS treatment, #p < 0.05, ##p < 0.01, and ###p < 0.001, compared with BMSCs, $p < 0.05, $$p < 0.01, and $$$p < 0.001, compared with EV-BMSCs, +p < 0. 05 and +++p < 0.001, compared with TuD-BMSCs by using two-tailed t test

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