Fig. 4

TRIAD1 co-localized with DISC1 in the primary NSCs. a Neurospheres were cultured as low-density cell suspensions in the presence of epidermal growth factor and basic fibroblast growth factor. And neurosphere was observed by a light microscope (a1, scale bar: 50 μm). Then, neurospheres were planted on coverslips and the expression of Nestin was detected (a2). b NSCs co-cultured with cm of LPS-astrocytes. Then immunofluorescence was performed to detect TRIAD1 (green, b2, b6; green, b18, b22), DISC1 (green, b10, b14; red, b19, b23), and NESTIN (red, b3, b7, b11, b15). The nuclei were stained with DAPI (blue, b1, b5, b9, b13, b17, b21). (b4) and (b8) show the co-localization of TRIAD1 (green) and NESTIN (red). (b12) and (b16) show the co-localization of DISC1 (green) and NESTIN (red). In (b20) and (b24), the merged images represent the co-localization of TRIAD1 and DISC1 in the nuclei of NSCs