Fig. 7

Cyclic hydrostatic pressure stimulation induces mineralisation of hBMSCs. A) Calcium concentration after 4 days of CHP + 10 days static culture (a) and 4 days of CHP + 17 days static culture (b). Alizarin S staining for mineralisation after long term pressure stimulation show presence of mineral nodules with CHP (4 days CHP + 17 days static culture) (c). Static—static condition in minimum osteogenic conditions, pressure mechanical stimulation in minimum osteogenic conditions at 10 kPa, 100 kPa and 300 kPa at 2 Hz frequency. Scale bar = 250 μm. *p < 0.05, **p < 0.01, ***p < 0.001