Table 2 Primers and experimental conditions used for qPCR analysis
Gene | Tm (°C) | Primer concentration (nM) | Sequence | PCR product size (bp) |
|---|---|---|---|---|
18S | 60 | 300 | 5′- ATCGGGGATTGCAATTATTC -3′ | 130 |
3′- CTCACTAAACCATCCAATCG -5′ | ||||
GAPDH | 60 | 300 | 5′- ACAGTTGCCATGTAGACC -3′ | 95 |
3′- TTTTTGGTTGAGCACAGG -5′ | ||||
COX2 | 60 | 400 | 5′- GGAGAAAAGGAAATGTCTGC -3′ | 186 |
3′- GTAGGCAGGAGAACATATAAC -5′ | ||||
RUNX2 | 60 | 400 | 5′- GCAGTATTTACAACAGAGGG -3′ | 112 |
3′- TCCCAAAAGAAGTTTTGCTG -5′ | ||||
OPN | 60 | 400 | 5′- GACCAAGGAAAACTCACTAC -3′ | 84 |
3′- CTGTTTAACTGGTATGGCAC -5′ |