Fig. 5

Identification of uhrf1 as a uniquely expressed proliferation gene using in vitro comparative RNA-seq. a UHRF1 immunofluorescence staining in regenerating deer antler tissue. b RM cells cultured with 30 nM uhrf1 siRNAs for 3 days exhibited decreased proliferation relative to mock-transfected control. c C3H10T1/2 cells stably transfected with uhrf1 exhibited increased proliferation relative to untransfected control and empty plasmid control. C3H10T1/2 cells stably transfected with uhrf1 maintained contact inhibition. Representative growth curves are shown. d C3H10T1/2 cells stably transfected with uhrf1 and cultured with 100 ng/mL BMP-2 for 6 days exhibited increased ALP activity relative to untransfected control and empty plasmid control. Scale bars as indicated. Data were from n = 3 isolates (an independent herd for antler immunofluorescence studies) or n = 3 independent experiments with nine replicates per group for uhrf1 knockdown and overexpression proliferation and osteoblast differentiation studies. Gray circles indicate observed data points. Error bars indicate SEM. Statistical significance as indicated