Fig. 1
From: Inhibition of autophagy promoted high glucose/ROS-mediated apoptosis in ADSCs
High glucose induced autophagy in ADSCs. ADSCs were cultured in high-glucose medium or normal-glucose medium for 6, 12, or 24 h. a, b Images showing the effects of high glucose on autophagy in fluorescent-mRFP-GFP-LC3-adenovirus-infected ADSCs. Green dots, autophagosomes; red dots, autolysosomes; yellow dots, autophagosomes. c–e Representative Western blot images showing the protein levels of LC3-I, LC3-II, ATG5, and Beclin1. β-actin was used as an internal control. Every experiment was repeated at least three times. Error bars indicate mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001)