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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Inhibition of autophagy promoted high glucose/ROS-mediated apoptosis in ADSCs

Fig. 1

High glucose induced autophagy in ADSCs. ADSCs were cultured in high-glucose medium or normal-glucose medium for 6, 12, or 24 h. a, b Images showing the effects of high glucose on autophagy in fluorescent-mRFP-GFP-LC3-adenovirus-infected ADSCs. Green dots, autophagosomes; red dots, autolysosomes; yellow dots, autophagosomes. ce Representative Western blot images showing the protein levels of LC3-I, LC3-II, ATG5, and Beclin1. β-actin was used as an internal control. Every experiment was repeated at least three times. Error bars indicate mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001)

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