Fig. 4
From: Inhibition of autophagy promoted high glucose/ROS-mediated apoptosis in ADSCs
High glucose-induced autophagy was mediated by the ROS/JNK signaling pathway. a, b Time course analysis of phosphorylated and total p38, ERK, JNK, and AKT protein expression in ADSCs cultured in high-glucose condition. β-actin was used as an internal control. c–e Cells were pre-treated with SP600125 (20 μg/ml) or NAC (5 mM) for 2 h and then cultured in high glucose for 24 h. Western blot analysis of JNK, p-JNK, LC3B, Becline-1, and ATG5 expression. f, g Cells were infected with mRFP-GFP-LC3 adenovirus. Confocal microscopic analysis is shown. Green dots, autophagosomes; red dots, autolysosomes; yellow dots, autophagosomes. The data are representative of three independent experiments. Error bars indicate mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001 vs control; ###P < 0.001 vs high glucose+SP600125; @@@P < 0.001 vs high glucose+NAC)