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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Inhibition of autophagy promoted high glucose/ROS-mediated apoptosis in ADSCs

Fig. 5

Inhibition of autophagy promoted high glucose-induced ROS production in ADSCs. a, b Cells were pre-treated with 3-MA for 24 h and then cultured in high-glucose medium for 24 h. Cells were infected with mRFP-GFP-LC3 adenovirus and observed under confocal microscope. Green dots, autophagosomes; red dots, autolysosomes; yellow dots, autophagosomes. c, d The level of DCF-sensitive ROS was measured by a flow cytometer. e, f Intracellular ROS generation was visualized under the fluorescence microscope. The scale bars represent 100 μm. Data are shown as means ± S.D. of three independent replicates (***P < 0.001 vs control; ###P < 0.001 vs high glucose; @@@P < 0.001 vs 3-MA)

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