Fig. 4
The effect of HIF-1α inhibitor KC7F2 on hypoxic preconditioning induced MSC. To investigate the role of HIF-1α inhibitor KC7F2 on hypoxic preconditioning induced MSC, we introduced KC7F2 (20 μM) for 24-h treatment and used WB analysis and immunofluorescence to test hypoxic preconditioning (1% O2)-induced MSCs CXCR4/7 and apoptosis caspase-3 change. a The effect of HIF-1α inhibitor KC7F2 on HIF-1α and CXCR4/7 protein expression with WB analysis in hypoxic preconditioning induced MSC. b The effect of HIF-1α inhibitor KC7F2 on apoptosis protein caspase-3 and cleaved-caspase-3 expression with WB analysis in hypoxic preconditioning induced MSC