Fig. 6
The effect of knock down of LincRNA-p21 on hypoxic preconditioning-induced MSC survival. To investigate the role of LincRNA-p21 on hypoxic preconditioning-induced MSC survival, we introduced shLincRNA-p21 and used hypoxic preconditioning (1% O2)-induced MSCs proliferation and apoptosis at 6 h, which were respectively tested by CCK8, trypan blue dye, and Annexin V-PE/7-AAD-stained flow cytometry method. a The effect of knock down of LincRNA-p21 on hypoxic preconditioning-induced MSC apoptosis with Annexin V-PE/7-AAD stained flow cytometry method at 6 h. b Early apoptotic MSC with knock down of LincRNA-p21 under hypoxic preconditioning at 6 h. c MSC proliferation with knock down of LincRNA-p21 under hypoxic preconditioning tested by CCK8 method at 6 h. d MSC proliferation with knock down of LincRNA-p21 under hypoxic preconditioning tested by trypan blue dye at 24 h. Results are mean ± SD (n = 3). *p < 0.05 vs. group hypoxia (+) shRNA-control (−) shLincRNA-p21 (−)