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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning

Fig. 7

The effect of knock down of MSC LincRNA-p21 on HIF-1α and CXCR4/7 under hypoxic preconditioning. To research the role of MSC LincRNA-p21 on HIF-1α and CXCR4/7 under hypoxic preconditioning, we used shLincRNA-p21 to test signaling change by RT-PCR, WB analysis, and co-immunoprecipitation method to test signaling change under hypoxic preconditioning (1% O2) at 24 h. a Three different knock-down ways of LincRNA-p21 on MSC under hypoxic preconditioning. b The effect of knock down of MSC LincRNA-p21 on HIF-1α mRNA relative expression under hypoxic preconditioning. c The effect of knock down of MSC LincRNA-p21 on HIF-1α, Hyp564 HIF-1α, and CXCR4/7 protein expression under hypoxic preconditioning. d The effect of knock down of MSC LincRNA-p21 on interaction between HIF-1α and VHL protein. Results are mean ± SD (n = 3). *p < 0.05 vs. group shRNA-control or comparison

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