Fig. 2

Effects of IL-1β in HUVECs on CXCL9 mRNA and CXCL9 ligand secretion. a HUVECs were stimulated with 100 ng/ml IL-1β at the time point of 3, 6, 12, and 24 h. The CXCL9 mRNA expression was detected using quantitative real-time PCR. b HUVECs were stimulated with IL-1β then the HUVEC conditioned medium was collected at 18 and 24 h. The CXCL9 ligand protein was detected by ELISA to quantify the release of CXCL9 in supernatants. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. *P < 0.05 versus control, **P < 0.01 versus control