Fig. 4

CXCL9 induces MSCs chemotaxis invasion and the effects of CXCR3 antagonist in CXCR3-mediated MSC chemotaxis invasion induced by IL-1β. a MSCs were plated on gelatin-coated coverslips in 6-well plates with a 0.5% agarose drop supplemented with different concentrations of CXCL9. After incubation for 24 h, CXCL9 directed chemotaxis of MSCs in agarose drop assay for chemotactic invasion. Scale bars = 500 μm. b Quantitative results showing the chemotaxis invasion ability of MSCs migrated under agarose drop. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. ***P < 0.001. c MSCs were pretreated with AMG-487 for 2 h and plated on coverslips in 6-well plates with a 0.5% agarose drop supplemented with 50 ng/ml CXCL9. After incubation for 24 h, CXCL9 directed chemotaxis of MSCs in agarose drop assay for chemotactic invasion. Scale bars = 500 μm. d Quantitative results showing the chemotaxis invasion ability of MSCs migrating under agarose drop. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. *P < 0.05, ***P < 0.001. e MSCs were pretreated or non-pretreated AMG-487 and stimulated with or without IL-1β for 30 min. After pre-activation, MSCs were plated on agarose drop supplemented with the presence or absence of 50 ng/ml CXCL9 for 24 h. Scale bars = 500 μm. f Quantitative results showing the chemotaxis invasion ability of MSCs migrated under agarose drop. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. *P < 0.05, ***P < 0.001