Fig. 5

Effects of CXCR3 antagonist in CXCR3-mediated MSC transendothelial migration induced by IL-1β. a MSCs were pretreated or non-pretreated AMG-487and stimulated with or without IL-1β for 30 min. After pre-activation, MSCs were plated on activated or non-activated HUVEC monolayer in the upper chamber and incubated in the presence or absence of 50 ng/ml CXCL9 in the lower chamber for 16 h. MSCs: dapi (blue) with cell tracker (green); HUVECs: dapi without cell tracker. Negative control: HUVECs only. Scale bars = 300 μm. b Quantitative results showing the transendothelial migration of MSCs to the lower chamber. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. *P < 0.05, ***P < 0.001