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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity

Fig. 1

Tri-lineage differentiation of mESC-FBs. Cells were differentiated for up to 4 weeks and were analyzed at the times indicated. a Upper panels: 2d, morphology of individual cells at low density after seeding for 2 days; 5d, monolayer formed from cells cultured for 5 days; 4w, oil droplets formed in adipocytes after the cells were cultured for 4 weeks. Bottom panels: cells differentiated for 3 weeks were fixed and stained with the indicated dyes. The cells were photographed under a phase contrast microscope after they were stained with TB (2d and 5d), Oil Red O, Alizarin Red, or Safranin O. The cells shown in 4w panel were live cells without staining. Scale bar = 100 μm. b Analysis of tri-lineage marker expression by RT-qPCR in the cells differentiated for 10 days and 20 days. The values in the control group represent the mRNA levels in cells before differentiation. Values are mean ± SD of an experiment performed in triplicate and repeated three times with similar results. *p < 0.05, compared with respective controls (Con)

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