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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity

Fig. 2

MSC marker expression and clonal growth of mESC-FBs. a Comparative analysis of MSC marker expression by RT-qPCR in mESC-FBs and BM-MSCs. Results are mean ± SD of three independent experiments. b Flow cytometry analysis of selected MSC markers in mESC-FBs. Con represent cells stained with isotype control antibodies. Results are representatives of three independent experiments. c Clonal growth of mESC-FBs. The cells in the plate were photographed with a digital camera (a) or cells in a single colony photographed under a phase contrast microscope (100×) after they were stained with TB. The graph illustrates growth curves of three representative clones. Values are mean ± SD of an experiment performed in triplicate and repeated two times with similar results

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