Fig. 4From: Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunityAnalysis of senescence, cell cycle proteins, growth factors, and chemokines. a Senescence analysis of mESC-FBs and BM-MSCs cultured for 7 days. Senescent cells were identified by β-Gal activity staining (blue color) and photographed under a phase-contrast microscope. Scale bar = 100 μm. b, c RT-qPCR analysis of cell cycle proteins (b), growth factors, and chemokines (c). The mRNA levels of the tested genes were determined from untreated mESC-FBs and BM-MSCs. Results are means ± SD of three to five independent experiments. *p < 0.05, compared between two cell typesBack to article page