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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity

Fig. 6

mESC-FBs are less sensitive to cytotoxicity of inflammatory conditions than BM-MSCs. a mESC-FBs and BM-MSCs were cultured under the indicated conditions for 4 days. The cells were fixed and stained with TB and photographed under a phase-contrast microscope, scale bar = 100 μm. b Quantitative analysis of relative numbers of cells under the indicated treatment conditions. Values are mean ± SD of cell numbers counted in three fields from a representative experiment under the indicated treatment conditions as described in a. *p < 0.05, compared with respective controls (Con). c RT-qPCR analysis of inflammatory cytokine expression in untreated mESC-FBs and RAW cells (left panel) and in LPS-stimulated RAW cells (right panel). Values are mean ± SD of results from three independent experiments. *p < 0.05, left panel compared between two cell types; right panel, compared with respective controls (Con)

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Stem Cell Research & Therapy

ISSN: 1757-6512