Fig. 6

Effect of nature collagen matrix on multilayer formation of urothelium of UC-induced USC. Urothelially induced USC developed urothelium with five to seven cell layers stained positive for AE1/AE3 onto a SIS matrix under dynamic culture, which is similar to urothelial cells. In contrast, USC treated with EGF or SMC/CM grew thinner layer and USC alone formed a single layer. In addition, the induced USC penetrated into the porous SIS matrix to a great depth to form well adhesion structure of cell-matrix. Scale bar = 50 μm. Abbreviations: USC = urine-derived stem cells, UC = urothelial cells, SMC = smooth muscle cells, CM = conditioned medium, UC/CM = urothelium conditioned medium SMC/CM = smooth muscle cell-conditioned medium EGF = epidermal growth factor