Table 3 Derivation of CEnCs from hPSCs
From: Corneal cell therapy: with iPSCs, it is no more a far-sight
Authors/year/reference number | Stem cell type | Derived cell type | Time line in Days | Culture conditions | Remarks |
|---|---|---|---|---|---|
Zhang et al./2014/86 | ESCs | CEnCs | 25 | D0–9: EBs was placed on ECM-coated substrate in basal medium D10–25: transwell culture in CEnCM | Report the derivation of CEnC-like cells hESCs through the POMP |
McCabe et al./2015/88 | ESCs | CEnCs | 10 | D0–3: ESCs cultured in ES medium + SMAD inhibitors (Noggin, SB43152). D4-D10: ES medium + PDGF + DKK2 | Global gene analysis revealed the ES-derived CEnCs similar to their in vivo counterparts |
Zhao et al./2016/31 | ESCs/iPSCs | CEnCs | D27 | D0–2: cultured in priming medium D3–9: EFSC generation D10–18: NCSC derivation D19–27: NCSCs differentiated to CEC | Generate CEnC from PSCs under defined culture conditions following a multi-step differentiation process |
Song et al./2016/83 | ESCs | CEnCs | 30 | D0–14: NCC induction D15–30: CEnC derivation | Compared transcriptome of ESC-derived CEnCs to in vivo counterpart |
Zhang et al./2017/87 | ESCs | CEN | 25 | D0–25: culture conditions as mentioned by Zhang et al. 2014 | Developed strategy for the construction of TECS by co-culturing ESC-derived LEC and CEnCs |