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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors

Fig. 1

The efficiency of synthetic mRNA transfection of PDX1 and NKX6.1 into SEES3 human ESCs. a Generation of synthetic messenger RNAs. ARCA: anti-reverse cap analog, pseudo-UTP: pseudouridine-5′-triphosphate, 5-Me-CTP: 5-methyl cytidine-5′-triphosphate. b Expression of synthetic messenger RNA for fluorescent proteins Emerald and mCherry in SEES3 human ESCs. Scale bars, 200 μm

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