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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors

Fig. 2

Schematic of differentiation protocol and characterization at day 3. a The differentiation protocol for human ESCs into pancreatic endocrine cells. The transfection schedule, growth factor, small chemical molecules, medium, and duration for each stage are shown. b Gene expression of PDX1 (n = 4) and NKX6.1 (n = 6) was analyzed by qRT-PCR at day 3. The Y axis indicates the relative change of mRNA expression compared with that of ES and no transfection (=1). Results were shown relative to the endogenous control GAPDH. *P < 0.05. Error bars represent SEM (n = 5). c Protein expression of PDX1 and NKX6.1 was analyzed by immunostaining at day 3. Scale bars, 50 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512