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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors

Fig. 3

Expression levels of pancreatic transcription factors at day 4. a Definitive endoderm (DE): FOXA2 (n = 5) and SOX17 (n = 6). b Primitive gut tube (PG): HNF1B (n = 5), posterior foregut endoderm: HNF6 (n = 6). c Pancreatic endoderm and endocrine precursor (PE): NGN3 (n = 6), PAX4 (n = 6), NKX2.2 (n = 6), and NEUROD1 (n = 6). Compared with ES, no transfection (=1) and transfection. *P < 0.05. N.S no significance. Error bars represent SEM (n = 5). d Immunofluorescence images of cells at day 3 for FOXA2, HNF1B, HNF4A, HNF6, and NEUROD1 all in green. Scale bars, 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512