Mapping of equine mesenchymal stromal cell surface proteomes for identification of specific markers using proteomics and gene expression analysis: an in vitro cross-sectional study

Table 2 Identified genes and/or proteins appointed to the cluster of differentiation (CD) classification system

Protein	Alternative protein name	Accession number	Gene name	AT-MSCs1	AT-MSCs2	AT-MSCs3	BM-MSCs1	BM-MSCs2	BM-MSCs3
CD29	Integrin subunit beta 1	F6UR46	ITGB1	+/+	+/+	+/+	+/+	+/+	+/+
CD44		Q05078	CD44	+/+	+/+	+/+	+/+	+/+	+/+
CD49a	Integrin subunit alpha 1	F6SHD8	ITGA1	+	+	+	+	+	+
CD49c	Integrin subunit alpha 3	F6W8D8	ITGA3	+	+	+	+	+	+
CD49d	Integrin subunit alpha 4	F6ZZX8	ITGA4	–	–	–	+	+	+
CD49e	Integrin subunit alpha 5	F6QLZ6	ITGA5	+	+	+	+	+	+
CD51	Integrin subunit alpha V	F6W3W7	ITGAV	+	+	+	+	+	+
CD56	Neural cell adhesion molecule 1	F7BT93	NCAM1	+	+	+	–	–	–
CD61	Integrin subunit beta 3	F7A370	ITGB3	+	+	–	+	+	+
CD71	Transferrin receptor 1	Q2V905	TFRC	+	+	+	+	+	+
CD73	Ecto 5′ nucleotidase	F6XEP5	NT5E	+	+	+	+	+	+
CD90	Thy-1 cell surface antigen	F6ZC75	THY1	+/+	+/+	+/+	+/+	+/+	+/+
CD91	Alpha-2-macroglobulin receptor	F6Q221	LRP1	+	+	+	+	+	+
CD105	Endoglin	F6W046	ENG	+/+	+/+	+/+	+/+	−/+	+/+
CD109		F6V1V8	CD109	+	+	+	+	+	+
CD142	Tissue factor	F6UIK6	F3	+	+	+	+	+	+
CD166	Activated leukocyte cell adhesion molecule	F7B5L3	ALCAM	+/+	+/+	+/+	+/+	+/+	+/+
CD228	Melanotransferrin	F7DGD6	MFI2	–	–	–	+	+	+
CD315	Prostaglandin F2 receptor inhibitor	F6WPE1	PTGFRN	+	+	+	+	+	+

Cells are isolated from samples harvested from three different horses (1–3). The cell surface proteins were enriched with biotinylation, and the samples analyzed by mass spectrometry. Raw data was searched against the Equus caballus reference sequence database from Uniprot (UP000002281; May 16, 2017; 22,698 proteins) giving information about protein names, accession numbers, and gene names. Gene expression was used to verify the data. (+) indicates that the protein was identified in the sample, (−) indicates that the protein was not identified in the sample. (+/+) indicates identification both on the protein and gene level. (−/+) indicates no identification on the protein level, but identification on the gene level
AT-MSCs adipose tissue-derived mesenchymal stromal cells, BM-MSCs bone marrow-derived mesenchymal stromal cells

ISSN: 1757-6512